57 years P = 0.049), as well as clinical severity scores, APACHE II (29.8 vs. The profile of death patients were men (64.7%, n = 22), with significantly higher average age (63 vs. The main sources of infection were respiratory tract 38% and intraabdomen 45% 70.7% had medical pathology. The beginning of severe sepsis took place in the emergency area in 46% of cases. The median age was 64 years old (interquartile range, 48.7 to 71) male: 60%. Results We analyzed 150 consecutive episodes of severe sepsis (16%) or septic shock (84%) admitted to the ICU. Descriptive andĬomparative statistical analysis was performed using SPSS version 15.0 (SPSS Inc., Chicago, IL, USA). PC activity was analysed using a haemostasis laboratory analyser (BCS® XP Siemens). Demographic, clinical parameters and coagulation markers during the first 24 hours were studied. 0.02 (0.015 0.044) |g/g/hour, P 18 years with severe sepsis according to the Surviving Sepsis Campaign, in an ICU of a university hospital. Results Adrenomedullin antagonism decreased the noradrenaline requirements needed to achieve target hemodynamics (0.009 (0.009 Ġ.012. Creatinine blood levels and clearance were assessed as surrogate for glomerular filtration. noradrenaline were titrated to maintain normotensive (mean blood pressure >60 mmHg) and hyperdynamic hemodynamics. Colloid fluid resuscitation and continuous i.v. Fifteen hours later animals were anesthetized, mechanically ventilated and instrumented for a consecutive 6-hour observation period. Results (1) No differences in SOFA scores were observed between adult sepsis (n = 19, 39 years) and older adult sepsis (n = 25, 78 years), but 3-month survival in older adult sepsis was significantly decreased compared with that in adult sepsis (36% vs. Values were compared among four groups: normal adult (65 years of age), adult sepsis (65 years of age) groups. Separated peripheral blood mononuclear cells were stained with CD4, CD8, programmed death-1 (PD-1), CD28, and CD62L antibodies and analyzed by flow cytometry, and serum was used to measure cytokine concentrations by using multiplex bead assay. (2) Blood samples were collected from septic and control volunteers. Results (1) Klotho septic mice started to die from 8 to 12 hours after CLP, and final survival of Klotho mice with CLP was significantly lower than that of WT with CLP (0% vs. Bacterial colony count in peritoneal lavage was also analyzed. Spleens, thymus, and serum were harvested for FACS analysis using caspase 3 as a marker for apoptosis, and blood for serum cytokine assay. (2) Cell analysis study: mice were sacrificed at 8 hours post CLP or sham surgery. Methods (1) Survival study: cecum ligation puncture (CLP) was performed to Klotho and wild-type (WT) mice and 4-day survivals were compared. The purpose of the study is to elucidate the immunological changes that occur in Klotho mice after sepsis in order to identify therapeutic targets for sepsis that occurs in aged individuals. Klotho knockout mice (Klotho mice) develop a syndrome resembling human aging, and exhibit shortened life spans (8 weeks) however, details regarding the immunity of and immunological changes in Klotho mice after sepsis are still unclear. Introduction Sepsis is primarily a disease of the aged and 60% of sepsis occurs in patients older than 65 years, 80% of deaths due to sepsis occur in this age group. S Inoue, K Suzuki-Utsunomiya, K Suzuki-Utsunomiya, T Sato, T Chiba, K HozumiĬritical Care 2012, 16(Suppl 1):P1 (doi: 10.1186/cc10608) Impaired innate and adaptive immunity of accelerated-aged Klotho mice in sepsis 32nd International Symposium on Intensive Care and Emergency Medicineīrussels, Belgium, 20-23 March 2012 Published: 20 March 2012
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